When natural barriers separate animals, they have to come up with new tactics of survival. They have to adapt to the new environmental conditions so that they can survive. The species will undergo through evolution and speciation would result. Due to the different existing conditions, each group will end up acquiring some genetic traits that may differentiate it from the other group.
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However, if they come into contact again, they will experience, reproduction isolation as a way of maintaining the traits that had already been acquired. The reproduction isolation will ensure that the species continue to evolve in their independent paths, by preventing interbreeding